Single-tube nested PCR in the diagnosis of tuberculosis.
نویسندگان
چکیده
منابع مشابه
Single-tube nested PCR in the diagnosis of tuberculosis.
AIMS To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity withou...
متن کاملSingle-tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis.
Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that bacterial mRNA has an extremely short half-life, averaging only a few minutes...
متن کاملNested PCR in lung tissue for diagnosis of pulmonary tuberculosis.
Diagnosis of pulmonary tuberculosis (TB) is difficult in cases with an unusual presentation and often requires a lung biopsy. The goal of this study was to determine the clinical usefulness of nested PCR on lung tissue for the diagnosis of pulmonary TB. Clinical and laboratory data were reviewed from patients who underwent diagnostic lung biopsies, followed by nested TB PCR on formalin-fixed pa...
متن کاملSingle-tube nested PCR with room-temperature-stable reagents.
Institut f~r Laboratoriumsund Transfusionsmedizin, Herzund Diabeteszentrum Nordrhein-Westfalen, Universit~itsklinik der Ruhr-Universit~it Bochum, Bad Oeynhausen, Germany The application of nested PCR in medical diagnostics implies that the same type of reaction is performed repetitively in the same laboratory over long periods of time, such as in the longitudinal monitoring of immunosuppressed ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1996
ISSN: 0021-9746
DOI: 10.1136/jcp.49.4.290